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Intel International Science and Engineering Fair (ISEF)

Individual Project Finalists

Name:Benjamin Wong Ngie Xiong
School:SMK Batu Lintang, Kuching

Project Title:Agro-Based Water Treatment for Rural Communities

Project Overview:
This study aims to research the possibility of using natural coagulants to precipitate out colloidal particles that causes turbidity in untreated water in rural areas.

The goal of the investigation is to help villagers to get clean water supply using natural and economical resources, and find natural coagulants without negative side effects and is safe to use.
Different natural coagulants will be tested to find out which gives the best results with the least cost incurred. In my study, I chose to use Jackfruit seeds, Ridge gourd and Breadnut seeds as these plant materials are readily available locally and are cheap. This makes the end product economically viable if marketed on a commercial scale.

My studies showed that Jackfruit seed powder (JFSP) is the most effective in treating turbid water. Therefore, I determined the optimum dosage of JSFP need to clean simulated turbid water.
Parameters of water quality tested include turbidity, conductivity, TDS, pH and time taken for turbid water to clear. The effectiveness of JFSP is than compared to Alum.

Furthermore, the use of JFSP as a coagulant aid was tested using raw river water collected from Rejang River, Sibu. One-Way ANOVA was conducted to see if there are any significant differences in the data obtained.

Results showed that 2.0g of JFSP is as effective as Alum in treating moderately turbid water to below safety standards set by WHO without lowering the pH levels.

I conclude that Jackfruit seed powder can be used as a coagulant and coagulant aid to reduce and replace the use of Alum.

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Name:Muhammad Danial Bin Ahmad Helmi
School:MARA Junior Science College Beseri, Beseri, Perlis

Project Title:Presence of Luciferase in Grass Prolongs Photosynthesis Process

Project Overview:
My project uses an extracted gene from a firefly, “luciferase gene”, with an aim to conserve Mother Nature.

To get the gene extracted, we must first undergo DNA Extraction [Miller et 1988]. The DNA is then tested with the Electrophoresis Technique using marker size 10 bp.

The gene is then purified and isolated to get a clean strand of DNA. I then conducted a Polymerase Chain Reaction (PCR) to create millions of copies of the gene.
The DNA extracted from 11 fireflies produces an amount of 5 microliter of DNA. The PCR process produces approximately 0.5 milliliter of the luciferase gene with the size of 3421 bp that has been extracted from 11 fireflies.

After the gene undergoes the PCR process, tissue culturing is used to insert the DNA. Once the specimen is fully inserted, the grass is left out under the sun.

The average photo intensity recorded using the Lux Meter is 1.06(lx). At night, the grass glows and undergoes a low rate of photosynthesis using the presence.

Having done this project, I believe that using the presence of the luciferase gene increases Photosynthesis in grass to produce better air quality and cut down on global warming.

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Name:Wan Muhd Syafiq Bin Wan Mohd Zairuddin
School:Mara Junior Science College, Taiping, Perak

Project Title:Kenaf Plant: A Green Source of Energy

Project Overview:
Kenaf or Hibiscus Cannanibus L, is a tropical fiber crop closely related to cotton and jute. The Kenaf core fiber has the potential to absorb domestic cooking oil waste.

This project was carried out to find the best type of sample to absorb the highest percentage of oil waste and to produce an environmental energy source, the Kenaf pallet.

In the processes, Kenaf sample in powder form was separated into two sizes, 255um and 125 um. Each size was tested for absorption of waste cooking oil, and went through a burn test and a thermogravimetric analyzer (TGA) test.

Results show that the Kenaf sample in 125um with water absorbs 86.24% of oil compared to the 355um sample that absorbs 84.96%. This is due to the fact that the 125um sample is more porous and has a higher surface area. The experiment without water from the 125um sample also yielded the highest percentage of oil absorption of 99.83%.

A Kenaf pallet prudced from 125um can burn longer compared to 355um. Results show that the Kenaf pallet produced using 125u metre by 0.5g can burn on average of 6 minutes compared to 355um that is 5 minutes.

Other than that, the thermogravimetric analyzer (TGA) test indicates less volatile matter produced by the Kenaf pallet than charcoal by 11.30%.

In conclusion, I believe that a Kenaf pallet can be used as a model to solve oil spill problems and be a source of green energy towards a better environment in the future.

Intel ISEF Malaysia
Finalists 2012